Modified 3′-End Amplification PCR for Gene Expression Analysis in Single Cells
نویسندگان
چکیده
منابع مشابه
Expression profiling of single cells using 3 prime end amplification (TPEA) PCR.
The ability to relate the physiological status of individual cells to the complement of genes they express is limited by current methodological approaches for performing these analyses. We report here the development of a robust and reproducible method for amplifying 3' sequences of mRNA derived from single cells and demonstrate that the amplified cDNA, derived from individual human lymphoblast...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03344bm06